Most melanomas are brought to a doctor’s attention because of signs or symptoms a person is having.

If an abnormal area of skin raises the suspicion of skin cancer, your doctor will use certain medical exams and tests to find out if it is melanoma, non-melanoma skin cancer, or some other skin condition. If melanoma is found, other tests may be done to determine if it has spread to other areas of the body.

Medical history and physical exam: Usually the first step is for your doctor to take your medical history. The doctor probably will ask when the mark on the skin first appeared, if it has changed in size or appearance, and if it has caused any symptoms (pain, itching, bleeding, etc.). You may also be asked about exposures to known causes of skin cancer (including sunburns) and if anyone in your family has had skin cancer.

During the physical exam, your doctor will note the size, shape, color, and texture of the area(s) in question, and whether there is bleeding or scaling. The rest of your body may be checked for spots and moles that could be related to skin cancer.

The doctor may also feel the lymph nodes (small, bean shaped collections of immune cells) under the skin in the groin, underarm, or neck near the abnormal area. When melanoma spreads, it often goes to nearby lymph nodes first. Enlarged lymph nodes might suggest that any melanoma present may have spread there.

If you are being seen by your primary doctor and melanoma is suspected, you may be referred to a dermatologist (a doctor who specializes in skin diseases), who will look at the area more closely.

Along with a standard physical exam, many dermatologists use a technique called dermatoscopy (also known asdermoscopy, epiluminescence microscopy [ELM], or surface microscopy) to see spots on the skin more clearly. The doctor uses a dermatoscope, which is a special magnifying lens and light source held near the skin. Sometimes a thin layer of oil is used with this instrument. The doctor may take a digital photo of the spot.

When used by an experienced dermatologist, this test can improve the accuracy of finding skin cancers early. It can also often help reassure you that a spot on the skin is likely benign (non-cancerous) without the need for a biopsy.

Skin biopsy: If the doctor thinks a spot may be a melanoma, he or she will take a sample of skin from the suspicious area to be looked at under a microscope. This is called a skin biopsy.

Different methods can be used for a skin biopsy. The doctor will choose one based on the size of the affected area, where it is on your body, and other factors. Any biopsy is likely to leave at least a small scar. Different methods may result in different types of scars, so ask your doctor about scarring before the biopsy is done. No matter which type of biopsy is done, it should remove as much of the suspected area as possible so that an accurate diagnosis can be made.

Skin biopsies are done using a local anesthetic (numbing medicine), which is injected into the area with a very small needle. You will likely feel a small prick and a little stinging as the medicine is injected, but you should not feel any pain during the biopsy.

Shave biopsy: For this type of biopsy, the doctor first numbs the area with a local anesthetic. The doctor then shaves off the top layers of the skin with a small surgical blade. Usually just the epidermis and the outer part of the dermis are removed, although deeper layers can be taken as well if needed. Bleeding from the biopsy site is then stopped by applying an ointment or a small electrical current to cauterize the wound.

A shave biopsy is useful in diagnosing many types of skin diseases and in sampling moles when the risk of melanoma is very low. A superficial shave biopsy is not generally recommended if a melanoma is suspected unless the shave biopsy sample goes deep enough to get below the suspicious area. Otherwise, it may not be thick enough to measure how deeply a melanoma has invaded the skin.

Punch biopsy: A punch biopsy removes a deeper sample of skin. The doctor uses a tool that looks like a tiny round cookie cutter. Once the skin is numbed with a local anesthetic, the doctor rotates the punch biopsy tool on the surface of the skin until it cuts through all the layers of the skin, including the dermis, epidermis, and the upper parts of the subcutis. The edges of the biopsy site are then stitched together.

Incisional and excisional biopsies: To examine a tumor that may have grown into the deeper layers of the skin, the doctor may use an incisional or excisional biopsy. After numbing the area with a local anesthetic, a surgical knife is used to cut through the full thickness of skin. A wedge or sliver of skin is removed for examination, and the edges of the wound are stitched together.

An incisional biopsy removes only a portion of the tumor. An excisional biopsy removes the entire tumor, and is usually the preferred method of biopsy for suspected melanomas if it can be done. But it is not always possible, so other types of biopsies may be needed.

Biopsies of melanoma that may have spread: Biopsies of areas other than the skin may be needed in some cases. For example, if melanoma has already been diagnosed on the skin, nearby lymph nodes may be biopsied to see if the cancer has spread that far (or farther).

In rare cases, biopsies may be needed to figure out what type of cancer someone has. For example, some melanomas may spread so quickly that they reach the lymph nodes, lungs, brain, or other areas while the original skin melanoma is still small. Sometimes these tumors are found before the melanoma on the skin is discovered. In other cases they may be found long after a skin melanoma has been removed, so it's not clear that it might be the same cancer.

In still other cases, melanoma may be found somewhere in the body without ever finding a spot on the skin. This may be because some skin lesions go away on their own (without any treatment) after some of their cells have spread to other parts of the body. Melanoma can also start in internal organs, but this is very rare, and if melanoma has spread widely throughout the body, it may not be possible to tell which tumor was the first one.

When it has spread like this, the metastatic melanoma in certain organs might be confused with a cancer starting in that organ. For example, melanoma that has spread to the lung might be confused with a primary lung cancer (cancer that starts in the lung).

Special tests can be done on the biopsy samples that can tell whether it is a melanoma or some other kind of cancer. This is important because different types of cancer are treated differently.

These types of biopsies may be more involved than those used to sample the skin.

Fine needle aspiration biopsy: A fine needle aspiration (FNA) biopsy is not used on suspicious moles, but it may be used to biopsy large lymph nodes near a melanoma to find out if the melanoma has spread to them. For this type of biopsy, the doctor uses a syringe with a thin, hollow needle to remove very small tissue fragments from a tumor. The needle is smaller than the needle used for a blood test. A local anesthetic is sometimes used to numb the area first. This test rarely causes much discomfort and does not leave a scar.

If the lymph node is near the body surface, the doctor can often feel it well enough to guide the needle into it. For a suspicious lymph node deeper in the body or a tumor in an internal organ such as the lung or liver, ultrasound or a computed tomography (CT) scan (a special type of x-ray; see “Imaging tests” below) is often used to guide the needle into place.

FNA biopsies are not as invasive as some other types of biopsies, but they may not always provide enough of a sample to tell if melanoma is present. In these cases, a more invasive type of biopsy may be needed.

Surgical (excisional) lymph node biopsy: This procedure can be used to remove an enlarged lymph node through a small skin incision. A local anesthetic is generally used if the lymph node is near the surface of the body, but a person may need to be sedated or even asleep (using general anesthesia) if the lymph node is deeper in the body.

This type of biopsy is often done if a lymph node’s size suggests the melanoma has spread but an FNA biopsy of the node was not done or did not find any melanoma cells.

Sentinel lymph node biopsy: If melanoma has been diagnosed and has any concerning features (such as being at least a certain thickness), a sentinel lymph node biopsy is often done to determine if it has spread to nearby lymph nodes, which in turn might affect treatment options. This test can be used to find the lymph nodes that are likely to be the first place the melanoma would go if it has spread. That is why these lymph nodes are called sentinel nodes (they stand sentinel, or watch, over the tumor, so to speak).

To find the sentinel lymph node (or nodes), the doctor injects a small amount of a radioactive substance (and sometimes a blue dye) into the area of the melanoma. After an hour or so, the doctor checks various lymph node areas with a radioactivity detector (which works like a Geiger counter). A small incision is made in the identified lymph node area. The lymph nodes are then checked to find which one(s) turned blue or became radioactive. These sentinel nodes are removed and looked at under a microscope.

If the sentinel node does not contain melanoma cells, no more lymph node surgery is needed because it is very unlikely the melanoma would have spread beyond this point. If melanoma cells are found in the sentinel node, the remaining lymph nodes in this area are removed and looked at as well. This is known as a lymph node dissection.

If a lymph node near a melanoma is abnormally large, a sentinel node biopsy may not be needed. The enlarged node is simply biopsied.

Lab tests of biopsy samples: Samples from any biopsies you have will be sent to a lab, where a pathologist (a doctor who is specially trained to diagnose disease) will look at them under a microscope for melanoma cells. Often, skin samples are sent to a dermatopathologist, a doctor who has special training in making diagnoses from skin samples.

If the doctor can’t tell for sure if the sample contains melanoma cells just by looking at it, special tests may be done on the cells to try to confirm the diagnosis. These tests have names such as immunohistochemistry (IHC),fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH).

If the samples do contain melanoma, the pathologist will look at certain important features such as the tumor thickness and mitotic rate (the portion of cells that are actively dividing). These features help determine the stage of the melanoma, which in turn affects treatment options and prognosis (outlook).

For people who have advanced melanoma, biopsy samples may be tested to see if the cells have mutations in theBRAF gene. About half of melanomas have BRAF mutations. Some newer drugs used to treat advanced melanomas are only likely to work if the cells have BRAF mutations 

Imaging tests: Imaging tests use x-rays, magnetic fields, or radioactive substances to create pictures of the inside of the body. They are used mainly to look for the possible spread of melanoma to lymph nodes or other organs in the body. They are not needed for people with very early-stage melanoma, which is very unlikely to have spread.

Imaging tests may also be done to help determine how well treatment is working or to look for possible signs of cancer recurrence after treatment.

Chest x-ray: This test may be done to help determine whether melanoma has spread to the lungs.

Computed tomography (CT) scan: The CT scan is a type of x-ray test that produces detailed, cross-sectional images of your body. Unlike a regular x-ray, CT scans can show the detail in soft tissues (such as internal organs). This test can help tell if any lymph nodes are enlarged or if organs such as the lungs or liver have suspicious spots, which might be due to the spread of melanoma. It can also help show spread to the lungs better than a standard chest x-ray.

Instead of taking one picture, like a regular x-ray, a CT scanner takes many pictures as it rotates around you. A computer then combines these pictures into detailed images of the part of your body that is being studied.

Before the scan, you may be asked to drink a contrast solution and/or get an intravenous (IV) injection of a contrast dye that helps better outline abnormal areas in the body. You may need an IV line through which the contrast dye is injected. The injection can cause some flushing (a feeling of warmth, especially in the face). Some people are allergic and get hives or, rarely, more serious reactions like trouble breathing and low blood pressure. Be sure to tell the doctor if you have any allergies or have ever had a reaction to any contrast material used for x-rays.

CT scans take longer than regular x-rays. You need to lie still on a table while they are being done. During the test, the table slides in and out of the scanner, a ring-shaped machine that completely surrounds the table. You might feel a bit confined by the ring you have to lie in when the pictures are being taken. Spiral CT (also known as helical CT), which uses a faster machine that yields more detailed pictures, is now used in many medical centers.

CT-guided needle biopsy: CT scans can also be used to help guide a biopsy needle into a suspicious area within the body. For this procedure, you remain on the CT scanning table while a radiologist moves a biopsy needle through the skin and toward the suspicious area. CT scans are repeated until the needle is in the mass. A needle biopsy sample is then removed and looked at under a microscope.

Magnetic resonance imaging (MRI): Like CT scans, MRI scans give detailed images of soft tissues in the body. But MRI scans use radio waves and strong magnets instead of x-rays. The energy from the radio waves is absorbed by the body and then released in a pattern formed by the type of body tissue and by certain diseases. A computer translates the pattern into a very detailed image of parts of the body. A contrast material might be injected, just as with CT scans, but is used less often.

MRI scans are very helpful in looking at the brain and spinal cord.: MRI scans take longer than CT scans – often up to an hour. You may have to lie inside a narrow tube, which is confining and can upset people with a fear of enclosed spaces. Newer, more open MRI machines can sometimes be used instead. The MRI machine also makes loud buzzing noises that you may find disturbing. Some places provide earplugs to help block this noise out.

Positron emission tomography (PET): For a PET scan, you receive an injection of a radioactive substance (usually a type of sugar related to glucose, known as FDG). The amount of radioactivity used is very low. Because cancer cells in the body are growing quickly, they absorb large amounts of the radioactive sugar. After about an hour, you are moved onto a table in the PET scanner. You lie on the table for about 30 minutes while a special camera creates a picture of areas of radioactivity in the body. The picture is not finely detailed like a CT or MRI scan, but it can provide helpful information about your whole body.

This test can be useful to see if the cancer has spread to lymph nodes. PET scans can also help when your doctor thinks the cancer has spread but doesn't know where. Doctors find it most useful in people with advanced stages of melanoma. It is not very helpful in people with early-stage melanoma.

Some newer machines are able to do both a PET and CT scan at the same time. This lets the doctor compare areas of higher radioactivity on the PET with the more detailed appearance of that area on the CT.

Blood tests: Blood tests aren't used to diagnose melanoma, but some tests may be done before or during treatment, especially for more advanced melanomas.

Doctors often test for blood levels of a substance called lactate dehydrogenase (LDH) before treatment. If the melanoma has spread to distant parts of the body, a higher than normal level of LDH is a sign that the cancer may be harder to treat. This affects the stage of the cancer.

Other tests of blood cell counts and blood chemistry levels may be done in a person who has advanced melanoma to monitor the function of the bone marrow (where new blood cells are made), liver, and kidneys during treatment.

How is melanoma skin cancer staged?